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Related Experiment Videos

Cell-substrate contacts in cultured chick embryonic cells: an interference reflection study

G W Ireland, C D Stern

    Journal of Cell Science
    |December 1, 1982
    PubMed
    Summary
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    Early chick tissue explants show dynamic cell-substrate contacts, forming broad adhesions and extracellular matrix. Specialized contacts develop faster in some tissues and are influenced by serum concentration.

    Area of Science:

    • Developmental Biology
    • Cell Biology
    • Tissue Engineering

    Background:

    • Cell-substrate interactions are crucial for tissue development and function.
    • Understanding early chick tissue spreading provides insights into fundamental developmental processes.

    Purpose of the Study:

    • To investigate the dynamics of cell-substrate contacts in explants from different regions of early chick tissues.
    • To characterize the formation and changes in cell-substrate adhesions and extracellular matrix during tissue spreading.

    Main Methods:

    • Interference reflection microscopy was employed to visualize cell-substrate contacts.
    • Explants from various chick tissue regions were cultured and analyzed over time.
    • Confrontation assays were performed to study cell interactions during invasion.

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    Main Results:

    • Explants spread as epithelial sheets with peripheral cells forming broad substrate contacts.
    • A fibronectin-containing extracellular matrix was observed beneath the explants.
    • Focal contacts and adhesions increased, associated with stress fibers, with varying kinetics across tissue types.
    • Serum concentration influenced the speed of specialized cell contact development.
    • Invading cells typically underlapped invaded cells, but invasion could occur even with established adhesions.

    Conclusions:

    • Cell-substrate contact formation is a dynamic process influenced by tissue origin and microenvironment (serum concentration).
    • The study elucidates the role of fibronectin and focal adhesions in early tissue spreading and cell behavior.
    • Findings contribute to understanding cell adhesion mechanisms relevant to tissue development and regeneration.