Development of the optic nerve in Xenopus laevis. I. Early development and organization

Development of the Xenopus laevis optic nerve was studied by light and electron microscopy from embryonic stage 26, before the retina has formed, to juveniles, 8 months postmetamorphic. Low-power EM photographs of sections through the retinal optic nerve (RON), middle optic nerve (MON) and chiasmatic optic nerve (CON) were prepared at different stages and the areas containing large axons (0.5 micron) were traced in optic nerve reconstructions. Ordering of fibre size along a dorsoventral axis was noted in the embryonic nerve, and this pattern persisted throughout development. Most large fibres, myelinated and unmyelinated, occupy an eccentric dorsocentral position in the MON while small axons are seen in a ventral peripheral crescent. In the CON, the dorsal one third to one half is occupied by large fibres while the ventral CON contains small fibres exclusively. If, as assumed, large axons are older than small axons (0.1-0.3 micron), then patterns of large and small axons along the nerve might reveal a chronotopic fibre ordering. Chronotopic ordering was confirmed by autoradiographic analysis of the distribution of old, labelled fibres and young, unlabelled newly arriving fibres in optic nerves between stage 51 and 57. The young-old labelling pattern corresponds to the small and large axon patterns respectively, in all sections of the optic nerve. Chronotopic ordering of fibres in the developing optic nerve can be explained, in part, by the dorsoventral asymmetric marginal growth of the developing retina and the phenomenon of fibre following as ganglion cell axons join near neighbour fascicles in the retina, converge at the optic disc and grow through the optic nerve.