Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterization of human complement components C6 and C7

R G Discipio, J Gagnon

    Molecular Immunology
    |November 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Immortalization of primary sheep embryo kidney cells.

    In vitro cellular & developmental biology. Animal·2021
    Same author

    Association between depression and hostile attribution bias in hostile and non-hostile individuals: An ERP study.

    Journal of affective disorders·2020
    Same author

    Integrating silver compounds and nanoparticles into ceria nanocontainers for antimicrobial applications.

    Journal of materials chemistry. B·2020
    Same author

    Synthesis, characterization, antibacterial activity and cytotoxicity of hollow TiO<sub>2</sub>-coated CeO<sub>2</sub> nanocontainers encapsulating silver nanoparticles for controlled silver release.

    Journal of materials chemistry. B·2020
    Same author

    Evaluation of the effect of vincristine on platelet count in dogs with lymphoma.

    The Journal of small animal practice·2019
    Same author

    Antibacterial activity of chemotherapeutic drugs against Escherichia coli and Staphylococcus pseudintermedius.

    Letters in applied microbiology·2019
    Same journal

    m6A modification of LINC00458 enhances HMOX1 stability via ELAVL1 recruitment to promote ferroptosis and aggravate asthma.

    Molecular immunology·2026
    Same journal

    Overexpression of Hes1 inhibits cigarette smoke-induced mitochondrial apoptosis in AT2 cells by activating the Pgc-1α/Tfam signaling pathway.

    Molecular immunology·2026
    Same journal

    Progesterone promotes favorable pregnancy outcomes in recurrent spontaneous, abortion by attenuating NK Cell overactivation and upregulating the cAMP/PKA/CREB signaling axis.

    Molecular immunology·2026
    Same journal

    Oleanolic acid alleviates hepatic fibrosis by inhibiting liver macrophage recruitment and polarization.

    Molecular immunology·2026
    Same journal

    Cordycepin attenuates diabetic nephropathy by dual-pathway activation of TFEB to restore autophagy and ameliorate podocyte injury.

    Molecular immunology·2026
    Same journal

    Endothelial-derived TWEAK drives granulosa cell apoptosis in PCOS via the Fn14-oxidative stress axis.

    Molecular immunology·2026
    See all related articles

    Researchers purified and characterized human complement components C6 and C7. These plasma proteins, C6 and C7, were found to be distinct single-chain proteins with different molecular weights and amino terminal residues, showing no sequence homology.

    Area of Science:

    • Immunology
    • Protein Chemistry

    Background:

    • The human complement system is crucial for innate immunity.
    • Understanding individual complement components is vital for elucidating system function.

    Purpose of the Study:

    • To purify and characterize human complement components C6 and C7.
    • To determine the biochemical properties and potential relationship between C6 and C7.

    Main Methods:

    • Protein purification techniques were employed for C6 and C7 isolation.
    • Molecular weight determination was performed.
    • Amino-terminal residue analysis was conducted.
    • Primary sequence analysis was utilized to assess homology.

    Main Results:

    • Human complement component C6 was purified as a single-chain plasma protein (104,800 Da) with 3.8% carbohydrate and alanine at the N-terminus.

    Related Experiment Videos

  • Human complement component C7 was purified as a single-chain plasma protein (92,400 Da) with 6.4% carbohydrate and serine at the N-terminus.
  • Primary sequence analysis revealed no evidence of homology between C6 and C7.

    • Conclusions:

    • Human complement components C6 and C7 are distinct proteins.
    • Biochemical characterization provides foundational data for further functional studies of C6 and C7.
    • The lack of homology suggests divergent evolutionary paths or specialized roles within the complement cascade.