Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Staphylococcal keratitis. Experimental model in guinea pigs

S D Davis, L D Sarff, R A Hyndiuk

    Archives of Ophthalmology (Chicago, Ill. : 1960)
    |November 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The association between gut microbiome and growth in infants with cystic fibrosis.

    Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2023
    Same author

    Comparative water relations of adjacent california shrub and grassland communities.

    Oecologia·2017
    Same author

    Tissue water relations of four co-occurring chaparral shrubs.

    Oecologia·2017
    Same author

    Water use patterns of four co-occurring chaparral shrubs.

    Oecologia·2017
    Same author

    Tissue water relations of three chaparral shrub species after wildfire.

    Oecologia·2017
    Same author

    Recovery patterns of three chaparral shrub species after wildfire.

    Oecologia·2017
    Same journal

    The economics of the initial preventive physical examination in medicare-reply.

    Archives of ophthalmology (Chicago, Ill. : 1960)·2013
    Same journal

    Modification of silicone oil retention sutures in aphakic eyes with iris loss-reply;.

    Archives of ophthalmology (Chicago, Ill. : 1960)·2013
    Same journal

    December 2011 archives web quiz winner.

    Archives of ophthalmology (Chicago, Ill. : 1960)·2013
    Same journal

    Angle involvement and glaucoma in patients with biopsy-proven iris melanoma: a response-reply.

    Archives of ophthalmology (Chicago, Ill. : 1960)·2013
    Same journal

    About this journal.

    Archives of ophthalmology (Chicago, Ill. : 1960)·2013
    Same journal

    In memoriam: goodwin m. Breinin, MD (1918-2011).

    Archives of ophthalmology (Chicago, Ill. : 1960)·2013
    See all related articles

    This study developed a guinea pig model for staphylococcal keratitis, finding early treatment with tobramycin or gentamicin sulfate highly effective against Staphylococcus aureus eye infections.

    Area of Science:

    • Ophthalmology
    • Microbiology
    • Infectious Diseases

    Background:

    • Staphylococcal keratitis is a serious eye infection.
    • Effective therapeutic strategies require robust experimental models for evaluation.

    Purpose of the Study:

    • To develop and validate an experimental model of staphylococcal keratitis in guinea pigs.
    • To quantitatively evaluate the efficacy of various antimicrobial agents against Staphylococcus aureus corneal infections.

    Main Methods:

    • Established a guinea pig model of staphylococcal keratitis using a virulent strain of Staphylococcus aureus.
    • Determined the bacterial growth curve in the cornea over time.
    • Assessed the efficacy of different topical antimicrobial treatments initiated at various time points post-infection.

    Related Experiment Videos

    Main Results:

    • Staphylococcus aureus rapidly multiplied in the cornea, peaking at 12 hours and declining after three days.
    • Therapy initiated 24 hours post-infection showed limited effectiveness.
    • Early treatment, especially at 8 hours or as prophylaxis, was significantly more effective.
    • Topical tobramycin sulfate and gentamicin sulfate (20 mg/ml) were superior to bacitracin, erythromycin, clindamycin phosphate, and a polymyxin B/neomycin/gramicidin combination when initiated at 8 hours.
    • Bacitracin and erythromycin ointments were ineffective.

    Conclusions:

    • Early intervention is critical for successful treatment of staphylococcal keratitis.
    • Topical tobramycin and gentamicin demonstrate superior efficacy in this experimental model.
    • The developed guinea pig model is suitable for quantitative assessment of anti-staphylococcal keratitis therapies.