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Experimental Bacteroides fragilis keratitis

G A Stern, E L Stock

Archives of Ophthalmology (Chicago, Ill. : 1960)

|December 1, 1978

Summary

This summary is machine-generated.

Bacteroides fragilis causes corneal abscesses in rabbits after intrastromal inoculation. These abscesses can persist and develop blood vessels, highlighting the need to consider anaerobic bacteria in keratitis diagnosis.

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Area of Science:

Ophthalmology
Microbiology
Infectious Diseases

Background:

Bacterial keratitis is a significant cause of vision loss.
The role of anaerobic bacteria, such as Bacteroides fragilis, in corneal infections is not fully understood.

Purpose of the Study:

To investigate the corneal pathogenicity of Bacteroides fragilis in a rabbit model.
To evaluate the clinical and histological progression of induced keratitis.

Main Methods:

Intrastromal inoculation of live Bacteroides fragilis organisms into rabbit corneas.
Clinical observation and histological examination of infected eyes over two weeks.
Assessment of bacterial recovery using anaerobic blood agar plates and thioglycollate broth.

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Main Results:

All intrastromal inoculations with live bacteria resulted in central corneal abscesses within 24 hours.
Abscesses persisted and showed vascularization in most observed eyes.
Superficial inoculation or inoculation with dead organisms did not induce lesions.
Both culture methods were effective for bacterial recovery, with broth cultures sometimes requiring longer incubation.

Conclusions:

Bacteroides fragilis is a potent corneal pathogen.
Anaerobic bacteria should be considered in the differential diagnosis of bacterial keratitis.
Specific laboratory methods are crucial for isolating anaerobic organisms from corneal infections.