Influence of the physiological regeneration and epithelialization using fractions isolated from Calendula officinalis

Standard skin wounds have been surgically induced in Wistar albino rats. The wounds were covered with 5% unguentum containing fractions C1 and C5, isolated from the flowers of Calendula officinalis belonging to fam. Compositae, in combination with allantoin. Epithelization has been determined in dynamics as a percentage compared with the beginning of the experiment, using the formula (formula: see text), where t is the wound surface in mm2 and n is the respective day after the beginning of the experiment. The wound exudate has been studied cytologically using light- and fluorescent microscopy on the 8th, 24th and 48th hour after inflicting the wounds. The histological changes in biopsy material taken from the edges of the wounds on the 10th day have also been investigated. The drug combination applied markedly stimulates physiological regeneration and epithelialization. This effect is assumed to be due to more intensive metabolism of glycoproteins, nucleoproteins and collagen proteins during the regenerative period in the tissues.