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Sialyltransferase activity in leukemia

R Sasaki, F Takaku, Y L Lin

    Leukemia Research
    |January 1, 1982
    PubMed
    Summary
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    Sialyltransferase activity is significantly altered in leukemia, being lower in acute lymphoblastic leukemia (ALL) and higher in acute non-lymphoblastic leukemia (ANLL) blasts. Enzyme activity may reflect leukocyte differentiation and malignant transformation.

    Area of Science:

    • Biochemistry
    • Hematology
    • Oncology

    Background:

    • Sialyltransferases are crucial enzymes involved in glycosylation.
    • Leukocyte differentiation and malignant transformation involve complex enzymatic changes.

    Purpose of the Study:

    • To investigate sialyltransferase activity in different types of leukemia.
    • To explore the relationship between sialyltransferase activity and leukocyte malignancy.

    Main Methods:

    • Enzyme activity assays were performed on blasts from acute lymphoblastic leukemia (ALL), acute non-lymphoblastic leukemia (ANLL), and chronic myeloid leukemia (CML) in blast crisis.
    • Activity was compared to normal lymphocytes and mature granulocytes.
    • Terminal deoxynucleotidyl transferase (TdT) activity was also measured.

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    Main Results:

    • Sialyltransferase activity was markedly lower in ALL blasts compared to normal lymphocytes.
    • Enzyme activity was significantly increased in ANLL blasts and TdT-negative CML blasts.
    • An inverse relationship was observed between sialyltransferase and TdT activity in TdT-positive ALL.

    Conclusions:

    • Sialyltransferase activity differs significantly across various leukemia subtypes.
    • Changes in sialyltransferase activity may serve as a marker for leukocyte differentiation and malignant transformation.