Use of a designed experiment for the study of muscle fibre size

Malignant Hyperpyrexia (MH) is the manifestation of an underlying muscle disease. In routine clinical examination of muscle biopsy tissue it had been noted that muscle fibre sizes appeared to differ between MH-susceptible individuals and non-susceptible individuals. A designed experiment was required to utilize data from the muscle samples at hand and to form the basis for a more general investigative study. The experiment and ensuing analysis are described and the results and their implications are discussed.