Effects in adipocytes of diamide on GSH levels, glucose uptake and cell integrity

Concentrations of insulin and chemical agents (H2O2, vitamine K-5) which stimulate hexose transport in fat cells do not alter the cellular levels of glutathione (reduced form; GSH). Diamide, another agent used in studies of insulin action, markedly reduces GSH levels and increases the movement of sugar into the cell. However, unlike insulin, H2O2 or vitamin K-5, diamide causes a change in the permeability of fat cells that allows entry of compounds (insulin, sucrose, L-glucose) which are normally excluded by the plasma membrane. Moreover, the accelerated rate of methylglucose uptake produced by diamide treatment is not inhibited by cytochalasin B, an agent that blocks basal and insulin-stimulated methylglucose transport. These results indicate that diamide does not cause a stimulation of the glucose transport system and should not be used (or used with caution) in transport studies. Furthermore, oxidation of GSH does not appear to be necessary for the stimulation of hexose transport in adipocytes by insulin, H2O2 or vitamin K-5.