Identification of human gene products from hybrid cells: a new approach

A method is described for detection of human polypeptides from hybrid cells, following high-resolution two-dimensional polyacrylamide gel electrophoresis of total cellular protein. 3H-labeled hybrid cell proteins are mixed with 14C-labeled parental cell proteins, electroporesis is carried out, and polypeptides that occur only in the 3H-labeled preparation are detected by double-label autoradiography. The possibility of developing a standard human polypeptide two-dimensional gel map, which would allow identification of electrophoretically separable components as specific proteins, is discussed.