[Polycytidylic matrix complex formation with complementary and noncomplementary purine nucleoside triphosphates]

Misincorportion into I poly(C) : I GTP complex, alpha = (formula: see text), has been determined by using H3-ATP. At ATP total concentration increasing from 6X10(-6) M to 4.5X10(-3) M the alpha value increases going asymptotically onto a plateau. The maximum misincorporation in the studied conditions is I adenilic resiude per congruent to 1400 guanilic ones. No ATP incorporation into the 2 poly(C) : I GTP complex was found. It is shown that misincorporation in non-enzymic recognition is essentially determined both by the difference in complementary and non-complementary monomer stacking and by the difference in their binding with the polynucleotide template.