Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Internal motions in deoxyribonucleic acid II

M E Hogan, O Jardetzky

    Biochemistry
    |July 22, 1980
    PubMed
    Summary

    DNA fragments exhibit significant internal motions in their base planes, sugar, and backbone. These fast, large-amplitude movements are crucial for understanding DNA helix dynamics.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Extent of intracellular pH changes during H(+) extrusion by maize root-tip cells.

    Planta·2013
    Same author

    Allelopathy of small everlasting (Antennaria microphylla) : Phytotoxicity to leafy spurge (Euphorbia esula) in tissue culture.

    Journal of chemical ecology·2013
    Same author

    Differential allelochemical detoxification mechanism in tissue cultures ofAntennaria microphylla andEuphorbia esula.

    Journal of chemical ecology·2013
    Same author

    Effect of temperature and sucrose concentration on hydroquinone toxicity in leafy spurge suspension culture cells.

    Journal of chemical ecology·2013
    Same author

    Varieties of positive and negative priming.

    Psychonomic bulletin & review·2013
    Same author

    The effect of ethidium bromide on DNA internal motions.

    Biophysical journal·2009

    Area of Science:

    • Molecular Biology
    • Biophysics
    • Nuclear Magnetic Resonance Spectroscopy

    Background:

    • Understanding the dynamic behavior of DNA is essential for comprehending its biological functions.
    • Internal motions within DNA structures influence molecular interactions and processes.

    Purpose of the Study:

    • To investigate the internal dynamics of deoxyribonucleic acid (DNA) fragments.
    • To quantify the amplitudes and timescales of motions within the DNA helix.

    Main Methods:

    • Nuclear Magnetic Resonance (NMR) spectroscopy was employed using 1H, 31P, and 13C nuclei.
    • Measurements were performed on monodisperse DNA fragments of 260 and 140 base pairs.
    • Temperature and solvent viscosity were varied to probe motional characteristics.

    Main Results:

    • Large-amplitude, fast internal motions were detected in the base planes, deoxyribose sugar, and sugar-phosphate backbone of DNA.
    • Specific amplitudes (A) and correlation times (τ) were determined for these motions.
    • Base plane motions: A = ±20°, τ = 1 x 10⁻⁹ s.
    • Deoxyribose sugar (2') motions: A = ±20° to ±33°, τ = 1 x 10⁻⁹ s.
    • Sugar-phosphate backbone (P-H) motions: A = ±27°, τ = 2.2 x 10⁻⁹ s.

    Conclusions:

    • The DNA helix exhibits significant internal flexibility.
    • These fast internal motions are inherent properties of the rodlike DNA structure.
    • The findings provide insights into the dynamic nature of DNA at the molecular level.

    Related Experiment Videos