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Microfilaments and cytoplasmic microtubules in cell division cycle mutants of Schizosaccharomyces pombe
Abstract:
The occurrence of axial cytoplasmic microtubules (25 nm in diameter) and of microfilaments (7 nm in diameter) associated in bundles just below the plasma membrane of the yeast Schizosaccharomyces pombe is described. Both types of cytoplasmic filamentous structures were present in the cell division cycle mutant cdc 12-112 of this fungus incubated for 6 h at the restrictive temperature of 35 degrees C. Microtubules and microfilaments probably function in septum formation and (or) in the volume-related control of the terminal phenotype of the mutant.
Insights
This study describes cytoplasmic microtubules and microfilaments in the yeast Schizosaccharomyces pombe. These structures are likely involved in cell division and growth regulation.
Area of Science:
- Cell Biology
- Mycology
- Cytoskeletal Dynamics
Background:
- The yeast Schizosaccharomyces pombe is a model organism for studying eukaryotic cell division.
- Cytoplasmic filamentous structures, including microtubules and microfilaments, play crucial roles in cellular processes.
- Understanding the organization and function of these structures is key to deciphering cell cycle regulation.
Purpose of the Study:
- To describe the occurrence and association of axial cytoplasmic microtubules and microfilaments in Schizosaccharomyces pombe.
- To investigate the presence of these cytoskeletal elements in a cell division cycle mutant (cdc 12-112) under restrictive conditions.
- To elucidate the potential roles of microtubules and microfilaments in septum formation and cell size control.
Main Methods:
- Light microscopy and electron microscopy techniques were employed to visualize cytoskeletal structures.
- Analysis of the yeast mutant cdc 12-112 incubated at the restrictive temperature (35°C) for 6 hours.
- Observation and characterization of cytoplasmic microtubules (25 nm diameter) and microfilaments (7 nm diameter) located beneath the plasma membrane.
Main Results:
- Axial cytoplasmic microtubules and associated microfilaments were observed in bundles just below the plasma membrane of Schizosaccharomyces pombe.
- Both microtubules and microfilaments were present in the cdc 12-112 cell division cycle mutant after incubation at the restrictive temperature.
- The findings indicate the co-existence and potential interaction of these two filament systems within the yeast cell.
Conclusions:
- Microtubules and microfilaments are integral components of the yeast cytoskeleton in Schizosaccharomyces pombe.
- These cytoskeletal elements are likely involved in the critical processes of septum formation during cell division.
- The study suggests a role for microtubules and microfilaments in regulating the cell's terminal phenotype, possibly related to cell volume control.