Related Experiment Video
Updated: Aug 19, 2026

Assessment of Morphine-induced Hyperalgesia and Analgesic Tolerance in Mice Using Thermal and Mechanical Nociceptive Modalities
Published on: July 29, 2014
Noradrenaline release from slices of the thalamus of normal and morphine-dependent rats
Abstract:
The effect of morphine on potassium-induced stimulation of (3H)-noradrenaline release from slices of the rat thalamus was investigated. The in vitro addition of morphine (10(-6)M) significantly depressed potassium-induced tritium overflow by 42% and this was prevented by the prior addition of naloxone (3 x 10(-6)M) to the medium. The stimulation-evoked overflow of tritium from slices of the thalamus of morphine-dependent rats was not significantly different from normal controls. Addition of naloxone (10(-5)M) 10 min before exposure of the tissues to 20 mM K+ significantly enhanced noradrenaline release from 'dependent' slices. The results suggest that the basic release mechanism may have adapted to the continuous presynaptic inhibition of release by morphine.
More Related Videos
04:11A Conditioned Place Preference Protocol for Measuring Incubation of Craving in Rats
Published on: November 6, 2018
09:54Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence
Published on: March 8, 2020
Related Concept Videos
Adrenergic Neurons: Neurotransmission
Synthesis: Catecholamine synthesis requires tyrosine, which is taken...
Opioid Receptors: Overview
Analgesia and Pain Management