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Antibody-induced antigen redistribution on murine mammary tumor cells
Syngeneic antitumor antibody induced the capping of tumor-associated surface antigens on murine mammary tumor cells. Immunofluorescence staining showed that capping was maximal after 2 approximately 4 hr, and then the capped antigen-antibody complexes disappeared. Radioimmunoassay, however, showed that the quantity of antigen-antibody complexes was the same before and after capping. This redistribution of surface antigens on the tumor cells had no effect on the time course of antibody-dependent macrophage-mediated tumor lysis (ADMC). Inhibition of antigen movement on the cell surface by glutaraldehyde treatment did not inhibit ADMC. These results suggest that syngeneic antitumor antibody induces the capping of surface antigens on murine mammary tumor cells, but that this dynamic redistribution of surface antigens is not correlated with the susceptibility of the cells to ADMC.
Syngeneic antitumor antibody induced the capping of tumor-associated surface antigens on murine mammary tumor cells. Immunofluorescence staining showed that capping was maximal after 2 approximately 4 hr, and then the capped antigen-antibody complexes disappeared. Radioimmunoassay, however, showed that the quantity of antigen-antibody complexes was the same before and after capping. This redistribution of surface antigens on the tumor cells had no effect on the time course of antibody-dependent macrophage-mediated tumor lysis (ADMC). Inhibition of antigen movement on the cell surface by glutaraldehyde treatment did not inhibit ADMC. These results suggest that syngeneic antitumor antibody induces the capping of surface antigens on murine mammary tumor cells, but that this dynamic redistribution of surface antigens is not correlated with the susceptibility of the cells to ADMC.