Serum LH concentrations in cyclic buffalo (Bubalus bubalis)

Area of Science:

• Veterinary Science
• Reproductive Biology
• Animal Endocrinology

Background:

• Understanding reproductive cycles is crucial for livestock management.
• Luteinizing hormone (LH) plays a key role in ovulation and conception.
• Seasonal variations can impact reproductive efficiency in buffaloes.

Purpose of the Study:

• To determine circulating LH levels in Murrah buffaloes during their estrus cycle.
• To investigate potential seasonal differences in LH peaks and their correlation with conception rates.

Main Methods:

• Radioimmunoassay (RIA) was used to measure LH levels.
• 14 Murrah buffaloes were monitored throughout their estrus cycle.
• LH levels were compared between hotter (June-August) and cooler (October-December) months.

Main Results:

• Peak circulating LH levels at estrus onset were similar in hotter (20.80 ng/ml) and cooler (21.24 ng/ml) months.
• High LH levels persisted for approximately 4 hours.
• Basal LH levels averaged 1-2 ng/ml throughout the cycle.
• Conception rates were higher in the cooler months (5/6) compared to the hotter months (2/8).

Conclusions:

• Seasonal differences in LH peak concentrations were not significant in Murrah buffaloes.
• Conception rates were notably higher during the cooler months, indicating potential seasonal influences beyond LH peak levels.
• Further research is needed to explore other factors affecting seasonal reproductive success in buffaloes.