Presence of binding component(s) for testosterone in rat liver cytosol

The existence of testosterone-binding component in the cytosol fraction of the male rat liver was shown. The binding ability of the component with testosterone was decreased by the addition of excess unlabeled steroids, but the steroid binding activity of the serum was not affected. The Scatchard plot indicated that the component has a moderate binding affinity with testosterone and the number of the binding sites of the component is limited.