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Concanavalin A capping in polymorphonuclear leukocytes

M Rister, G Brucke, F Wegener

    European Journal of Pediatrics
    |December 1, 1980
    PubMed
    Summary
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    Polymorphonuclear leukocyte (PMN) function relies on their cytoskeleton. Bacterial infections and immunosuppressive therapy in children significantly increase spontaneous PMN capping, indicating microtubule assembly defects and altered leukocyte function.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Polymorphonuclear leukocyte (PMN) functions are critically dependent on an intact intracellular cytoskeleton, comprising microtubules and microfilaments.
    • The microtubule system plays a crucial role in various leukocyte functions.

    Purpose of the Study:

    • To investigate the microtubule system in PMNs by examining spontaneous and induced cap formation.
    • To assess alterations in PMN microtubule assembly in children with bacterial infections, viral infections, and those on immunosuppressive therapy.

    Main Methods:

    • Fluorescence microscopy was used to observe spontaneous, Colchicine, and Diamide-induced cap formation in PMNs.
    • PMNs were obtained from healthy controls, children with bacterial infections, children with viral infections, and patients on immunosuppressive therapy.

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    Main Results:

    • Children with bacterial infections showed a significantly increased number of spontaneous capped PMNs (47% +/- 1%) compared to controls (22% +/- 1%).
    • PMNs from patients on immunosuppressive therapy also exhibited a high rate of spontaneous capping (52% +/- 2%).
    • Colchicine and Diamide treatments increased cap formation in PMNs from both controls and patients, suggesting intact drug-induced responses despite altered spontaneous capping.

    Conclusions:

    • Increased spontaneous cap formation in PMNs is associated with defects in microtubule assembly.
    • Leukocyte function defects observed in patients with bacterial infections, bronchial asthma, or on immunosuppressive therapy may result from an altered microtubule system.