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An optically clear hypercholesterolemic hypertriglyceridemic quality-control material prepared from animal lipid
Clinical Chemistry
|March 1, 1981
Summary
This study details a novel method for creating a hyperlipidemic control serum using animal lipid sources. The resulting serum is clear, stable, and precise for laboratory use.
Area of Science:
- Biochemistry
- Clinical Chemistry
Background:
- Hyperlipidemic control serum is essential for accurate laboratory diagnostics.
- Existing methods for serum preparation may have limitations in stability or purity.
Purpose of the Study:
- To develop a simple, stable, and precise hyperlipidemic control serum from animal sources.
- To ensure the absence of hepatitis-associated constituents.
Main Methods:
- Porcine serum was treated with dextran sulfate and calcium ions to remove beta- and pre-beta-lipoproteins.
- A triglyceride-rich fraction was isolated from chicken egg-yolks.
- Fractions were combined and stabilized with Triton X-100 and sucrose.
Main Results:
- A clear, homogeneous, and stable hyperlipidemic control serum was successfully prepared.
- The serum demonstrated stability for long-term storage at 5°C and resistance to short-term freezing.
- The preparation was free of hepatitis-associated constituents.
Conclusions:
- This novel animal-derived serum offers a precise and stable alternative for hyperlipidemic control.
- The preparation method is simple and yields a high-quality diagnostic reagent.