Three-dimensional reconstruction from images of tilted specimens: the paramyosin filament

The three-dimensional structure of the paramyosin filament has been reconstructed from images obtained when the specimen is tilted at different angles about its long axis. The micrographs were correlated spatially using gold marker beads and digitized. The different views contained in each micrograph were combined using a computational technique involving Fourier transforms and the resulting three-dimensional images are presented as various sets of serial sections. The results confirm that the Bear-Selby (1956) net can be seen at all levels in the specimen.