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Updated: Aug 9, 2026

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A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood
Published on: April 28, 2016
[Serum amino acid determination after different blood processing and storage methods]
Summary
Blood sample storage for amino acid analysis is reliable for months at -18°C or below. Room temperature is acceptable for short processing times, ensuring accurate amino acid spectra results.
Area of Science:
- Biochemistry
- Analytical Chemistry
Context:
- Accurate amino acid analysis is crucial for diagnosing metabolic disorders and monitoring patient health.
- Standardized protocols for blood sample handling and storage are essential for reliable analytical results.
Purpose:
- To evaluate the impact of different storage temperatures and durations on the amino acid profiles of blood samples.
- To determine the stability of amino acids in blood samples under various pre-analytical conditions.
Summary:
- Blood samples were subjected to 39 preparation methods, including storage at temperatures from -78°C to -4°C for 2-6 months.
- Storage at -18°C or below for several months did not affect amino acid spectra, while -4°C showed slightly decreased glutamine levels.
- Blood samples can be processed at room temperature for up to 60 minutes without compromising analytical outcomes.
Impact:
- Establishes guidelines for blood sample storage, enabling extended analysis periods and improving laboratory workflow.
- Ensures the integrity of amino acid measurements, critical for clinical diagnostics and research.
- Reduces the need for immediate processing, offering flexibility in sample handling for amino acid analysis.

