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[Relation of mammalian cell resistance to actinomycin D with a change in the karyotype and a decrease in cytoplasmic
Genetika
|January 1, 1981
Summary
Djungarian hamster cells resistant to actinomycin D (AD) showed decreased drug uptake, indicating reduced membrane permeability. This resistance was unstable and linked to chromosomal abnormalities, suggesting gene amplification.
Area of Science:
- Cell Biology
- Genetics
- Pharmacology
Context:
- Cell lines selected for resistance to actinomycin D (AD) were studied.
- Resistance levels were 1000-4000 times higher than parent cells.
Purpose:
- To investigate the mechanism of actinomycin D resistance in Djungarian hamster cells.
- To explore the relationship between drug resistance and chromosomal alterations.
Summary:
- Resistant cell lines exhibited significantly reduced uptake of AD and colchicine, suggesting decreased plasma membrane permeability.
- The acquired AD resistance was unstable, diminishing upon removal of the drug.
- Chromosomal analysis revealed homogeneously staining regions (HSR) in resistant lines, supporting a link to gene amplification.
Impact:
- Provides evidence for the role of gene amplification in conferring drug resistance.
- Highlights the potential for developing strategies to overcome drug resistance in cancer therapy.
- Contributes to understanding cellular mechanisms of multidrug resistance.