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Methionine-defective mutants in Candida utilis

J M Delgado, A Alonso, X Alvarez

    Folia Microbiologica
    |January 1, 1981
    PubMed
    Summary

    Researchers developed ethionine-resistant mutants of Candida utilis that overproduce methionine. These mutants showed decreased intracellular methionine during stationary phase and altered methionine uptake, impacting their ability to use it as a nitrogen source.

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    Area of Science:

    • Microbiology
    • Biochemistry
    • Yeast Genetics

    Background:

    • Candida utilis is a yeast with metabolic pathways relevant to amino acid production.
    • Methionine is an essential amino acid with roles in cellular metabolism and protein synthesis.
    • Ethionine resistance can be a marker for altered amino acid metabolism in yeast.

    Purpose of the Study:

    • To isolate and characterize ethionine-resistant mutants of Candida utilis.
    • To investigate the metabolic alterations, particularly methionine uptake and utilization, in these mutants.
    • To identify the genetic basis for altered methionine metabolism in Candida utilis.

    Main Methods:

    • Isolation of ethionine-resistant mutants from Candida utilis CCY-158.
    • Cultivation of wild-type and mutant strains under various conditions.
    • Measurement of intracellular methionine concentrations.
    • Analysis of methionine utilization as a nitrogen and carbon source.
    • Identification of mutants with impaired methionine uptake (Mec- mutants).

    Main Results:

    • Ethionine-resistant mutants overproducing methionine were successfully isolated.
    • Intracellular methionine levels decreased significantly in mutants during the stationary phase.
    • Mutants unable to utilize methionine as a nitrogen source (Mec- mutants) were identified, linked to impaired methionine uptake.
    • Some Mec- mutants also showed reduced ability to utilize other amino acids as nitrogen sources.

    Conclusions:

    • Ethionine resistance in Candida utilis is associated with altered methionine metabolism and uptake.
    • Methionine uptake is a critical factor for its utilization as a nitrogen source in yeast.
    • These mutants provide valuable tools for studying amino acid transport and metabolism in Candida utilis.

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