Preparation of reference sera with desired levels of cholesterol and triglyceride

Simple methods for isolating cholesterol-rich protein fraction from human serum and triglyceride-rich fraction from hen egg yolk are presented. The isolated fractions are combined separately with small volumes of human serum in order to stabilize concentrated cholesterol and triglyceride. Precalculated volumes of concentrates are added to human serum in order to prepare reference sera with desired levels of cholesterol or triglyceride, or both. The reference sera resemble human serum in appearance, composition, and stability. Properties of preparations are discussed and stability of reference sera is illustrated.