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Related Experiment Videos

Brain clathrin: studies of its ultrastructural assemblies

M P Lisanti, W Schook, N Moskowitz

    European Journal of Biochemistry
    |January 1, 1982
    PubMed
    Summary

    Clathrin, a protein involved in cell structure, forms baskets or cages. Associated proteins influence clathrin assembly, and their removal alters its polymerization and structure formation capabilities.

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    Area of Science:

    • Cell Biology
    • Biochemistry
    • Structural Biology

    Background:

    • Clathrin is a protein known for its role in forming coated vesicles and intracellular trafficking.
    • Clathrin self-assembles into polyhedral lattices, cages, or baskets.

    Purpose of the Study:

    • To investigate the role of clathrin-associated proteins in clathrin assembly and structure formation.
    • To determine how chemical treatments and cytoskeletal-disrupting agents affect clathrin polymerization.

    Main Methods:

    • Purification of clathrin and analysis of associated polypeptides.
    • pH-induced assembly of clathrin into baskets/cages.
    • Chymotrypsin treatment to cleave associated proteins.
    • Turbidity measurements to quantify polymerization.

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  • Assessment of clathrin interactions with actin and alpha-actinin.
  • Treatment with cytoskeletal-disrupting agents (chlorpromazine, imipramine, colchicine, cytochalasin B, vinblastine).

    • Main Results:

    • Purified clathrin contains associated polypeptides that are essential for basket/cage formation.
    • Cleavage of associated proteins by chymotrypsin prevents basket formation but allows polymerization into open lattices.
    • Open lattice formation results in higher turbidity than basket formation.
    • Salts inhibit turbidity increase in both native and treated clathrin.
    • Chlorpromazine and imipramine increase turbidity without basket formation; colchicine and cytochalasin B have no effect.
    • Vinblastine causes clathrin precipitation but does not inhibit subsequent polymerization.

    Conclusions:

    • Clathrin exists in a complex with clathrin-associated proteins that regulate its polymerization extent and structure formation.
    • Associated proteins are crucial for the specific assembly of clathrin into baskets/cages.
    • Clathrin's polymerization dynamics and structural outcomes can be modulated by associated proteins and external agents.