Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microcirculation of transplanted tumors

B Endrich, K Messmer

    Arzneimittel-Forschung
    |January 1, 1981
    PubMed
    Summary

    Melanoma growth increases intratumor pressure and thrombosis, leading to higher capillary resistance. This enhanced resistance may reduce the effectiveness of melanoma treatments by impairing blood flow.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [DRG exercises: blunt abdominal trauma].

    Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2008
    Same author

    [DRG practice: wound management with vacuum therapy].

    Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2008
    Same author

    [DRG exercises: gastrectomy].

    Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2008
    Same author

    [DRG practice: Frequently asked questions--questions from general practice].

    Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2007
    Same author

    [In Process Citation].

    Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2007
    Same author

    [DRG exercises: Conservative and surgical treatment of humeral head fractures].

    Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2007

    Area of Science:

    • Oncology
    • Microcirculation Research
    • Cancer Biology

    Background:

    • Amelanotic melanoma (A-Mel-3) is a model for studying tumor microenvironment.
    • Understanding tumor vascular dynamics is crucial for effective cancer therapy.

    Purpose of the Study:

    • To investigate the impact of melanoma development on local tissue oxygenation, microvascular morphology, and capillary hemodynamics.
    • To assess the relationship between microcirculatory changes and potential therapeutic efficacy.

    Main Methods:

    • Implantation of transparent chambers in Syrian gold hamsters to allow in vivo microscopy of dorsal skin flap tumors.
    • Utilized platinum multiwire electrodes for local partial pressure of oxygen (pO2) measurements.
    • Employed quantitative video-techniques to analyze microvascular morphology, capillary perfusion, and blood cell velocity.

    Main Results:

    • Mean local pO2 decreased with tumor progression.
    • Neovascularization initially increased capillary density, followed by a significant decrease by day 12 due to increased intratumor pressure and thrombosis.
    • Platelet conglomerates in capillaries at the melanoma edge prolonged capillary filling time, indicating increased capillary resistance.

    Conclusions:

    • Melanoma development leads to increased intratumor pressure and thrombosis, resulting in elevated capillary resistance.
    • Enhanced capillary resistance may significantly impede melanoma treatment efficacy.
    • Therapeutic strategies for melanoma should consider the impact of microcirculatory changes and increased tissue pressure.

    Related Experiment Videos