Diurnal variation in release of LH and testosterone in the ram

The time of appearance of plasma LH and testosterone peaks through the day determined in 75 Préalpes du Sud and 41 Ile-de-France rams in December and in 44 Préalpes du Sud and 11 Ile-de-France rams in June. The distribution of peaks throughout the day was non-random for the two hormones in the two breeds and for both times of the year (P less than 0.01 at least on each occasion; P less than 0.001 on pooled data from the two breeds). The most striking features were the occurrence of (1) a minimum of LH and testosterone peaks immediately after 'dawn' (lights on) in both months; (2) a maximum of peaks 3 h after 'dawn' in June and 4 h after 'dawn' in December. For several hours after the increase in frequency of peaks the probability of measuring peaks of LH values in December and June when adjusted for the time of 'dawn' suggest that dawn could act as a synchronizer of gonadotroph activity.