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Related Experiment Videos

Novel vesicular extrusions during cell spreading

R Rajaraman, B Westermark, J Ponten

    Experientia
    |May 15, 1982
    PubMed
    Summary
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    Human cells release vesicular material during late-stage spreading, a process involving cytoskeletal elements and potentially removing damaged components.

    Area of Science:

    • Cell Biology
    • Cytoskeletal Dynamics
    • Extracellular Vesicle Biology

    Background:

    • Cell spreading is a fundamental process in cell biology.
    • Cells exhibit dynamic changes in their surface and internal structures during spreading.
    • The release of vesicular material from cells is not fully understood.

    Purpose of the Study:

    • To investigate the phenomenon of vesicular material extrusion during late-stage cell spreading.
    • To identify the cellular mechanisms and components involved in this extrusion process.
    • To explore the potential function of this extrusion in cellular maintenance.

    Main Methods:

    • In vitro culture of human glia, glioma cells, and diploid fibroblasts (MRC-5, WI-38).
    • Observation of vesicular material extrusion during cell spreading.

    Related Experiment Videos

  • Treatment with cytochalasin-B and colcemid to assess the involvement of microfilaments and microtubules.
  • Analysis of the extrusion mechanism in relation to known cellular processes like patching, capping, and endocytosis.

    • Main Results:

    • Vesicular material extrusion was consistently observed in late-stage spreading of tested human cell types.
    • The extrusion process was significantly inhibited by cytochalasin-B and colcemid.
    • These findings suggest the active involvement of both microfilaments and microtubules in the phenomenon.
    • The mechanism appears analogous to cellular processes involved in surface component remodeling.

    Conclusions:

    • Late-stage cell spreading involves the active extrusion of vesicular material.
    • Microfilaments and microtubules play crucial roles in mediating this extrusion.
    • This process may represent a mechanism for cells to shed damaged surface components.