Enhancement of human chorionic gonadotrophin production by antimetabolites

The action of methotrexate, actinomycin D, bleomycin, vincristine and hydroxyurea on the production of human chorionic gonadotrophin (hCG) by a choriocarcinoma cell line (BeWo) has been studied. hCG production per unit of cell protein was increased, and this was a continuing process only halted by cell death. The proportion of hCG-producing cells in the population increased from approximately 5% to a maximum of approximately 40% during incubation with vincristine. A possible explanation for these and related observations is that cytotoxic agents promote the differentiation of cytotrophoblast to syncytium. A similar mechanism could contribute to the unusual sensitivity of choriocarcinoma to cytotoxic agents.