Crabtree effect induced by fructose in hepatocytes isolated from regenerating liver

The inhibition of respiration caused by fructose is investigated in hepatocytes isolated from regenerating liver. After partial hepatectomy, decreased respiratory capacity of the cells was observed in spite of significantly enhanced levels per milligram of protein of cytochromes b and aa3 in the mitochondrial fraction. During the first few days after operation fructose did not affect the oxygen uptake. From the 10th day after surgery incubation with 10 mmol/l fructose caused an increase in the glycolytic flux parallel with an inhibition of respiration.