Binding of digitoxin, digoxin and gitoxin to human serum albumin

The binding of three digitalis glycosides, digitoxin, digoxin and gitoxin, to human serum albumin was studied in vitro by equilibrium dialysis. The results obtained showed that binding is a non saturable process and that probably the same binding mechanism is involved for each of the three drugs. Their binding sites seem to be different from of those of acidic and basic drugs. However, the three drugs were found to be partially displaced by large amounts of fatty acids.