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Interaction between cadmium and selenium in rat plasma

T A Gasiewicz, J C Smith

    Environmental Health Perspectives
    |August 1, 1978
    PubMed
    Summary
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    Selenium metabolism in rat red blood cells is dependent on glutathione (GSH). This process forms a selenium compound that interacts with cadmium, potentially altering cadmium

    Area of Science:

    • Biochemistry
    • Toxicology
    • Cell Biology

    Background:

    • Selenium (SeO3 2-) metabolism and its interaction with cadmium (Cd) in biological systems are not fully understood.
    • Erythrocytes play a role in the transport and metabolism of various compounds, including potential toxic metals.
    • Glutathione (GSH) is a critical cellular antioxidant involved in numerous metabolic pathways.

    Purpose of the Study:

    • To investigate the metabolism of selenite (SeO3 2-) by rat erythrocytes in vitro.
    • To determine the role of reduced glutathione (GSH) in selenite uptake and conversion.
    • To characterize the selenium-containing product formed and its interaction with cadmium and plasma proteins.

    Main Methods:

    • Incubation of (75)Se-labeled selenite with intact rat erythrocytes under varying conditions.

    Related Experiment Videos

  • Manipulation of erythrocyte reduced glutathione (GSH) concentrations using excess selenite and N-ethylmaleimide.
  • Analysis of selenium release, glutathione transport, and formation of cadmium-selenium complexes using gel-filtration and ion-exchange chromatography.

    • Main Results:

    • Selenite uptake and metabolism by erythrocytes are dependent on reduced glutathione (GSH), with glutathione selenotrisulfide (GSSeSG) as a probable intermediate.
    • Selenium release from erythrocytes is linked to glutathione reductase activity and is inhibited by chromate.
    • A cadmium-selenium complex formed in vitro is indistinguishable from that formed in vivo, suggesting a role for erythrocyte metabolism in Cd-Se interactions.

    Conclusions:

    • Rat erythrocytes metabolize selenite via a GSH-dependent pathway, producing a selenium compound (possibly H(2)Se) that complexes with cadmium.
    • Glutathione reductase appears to be involved in the secondary release of selenium from erythrocytes.
    • This erythrocyte-mediated selenium metabolism may influence the tissue distribution and toxicity of cadmium.