Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Neuronal cell cultures as toxicologic test systems

P G Nelson

    Environmental Health Perspectives
    |October 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Neuronal cell cultures offer a robust model for evaluating toxicant effects on developing neural networks. These systems allow for detailed analysis of synaptic function and neurochemical changes, crucial for understanding neurotoxicity.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Balancing a retroreflector to minimize rotation errors using a pendulum and quadrature interferometer.

    Applied optics·2015
    Same author

    On the intensity distribution function of blazed reflective diffraction gratings.

    Journal of the Optical Society of America. A, Optics, image science, and vision·2014
    Same author

    Innervation of the placenta and uterus: competition between cytotrophoblasts and nerves?

    Placenta·2013
    Same author

    Glia cell line-derived neurotrophic factor regulates the distribution of acetylcholine receptors in mouse primary skeletal muscle cells.

    Neuroscience·2004
    Same author

    Glial-neurotrophic mechanisms in Down syndrome.

    Journal of neural transmission. Supplementum·2002
    Same author

    Opposing actions of protein kinase A and C mediate Hebbian synaptic plasticity.

    Nature neuroscience·2001
    Same journal

    A New Start.

    Environmental health perspectives·2026
    Same journal

    Time-Varying Exposure to Element Mixtures and Children's Cognition at 5 Years of Age: Findings from the New Hampshire Birth Cohort Study.

    Environmental health perspectives·2026
    Same journal

    Effect of Household Air Pollution on the Gut Microbiome and Virome of Adult Women Living in Uganda.

    Environmental health perspectives·2026
    Same journal

    Comparison of Temperature-Mortality Associations across the Middle East Using Different Exposure Estimation Approaches.

    Environmental health perspectives·2026
    Same journal

    Workflow for Statistical Analysis of Environmental Mixtures.

    Environmental health perspectives·2026
    Same journal

    Effects of Extreme Heat Exposure on Heatstroke and Liver Injury in Mice: The Role of PPARα.

    Environmental health perspectives·2026
    See all related articles

    Area of Science:

    • Neuroscience
    • Toxicology
    • Cell Biology

    Background:

    • Neuronal cell cultures are established models for studying toxicological effects.
    • Synapse formation and development occur over days to weeks in these cultures.
    • Developing neurons and synapses are particularly susceptible to harmful agents.

    Purpose of the Study:

    • To evaluate the utility of neuronal cell cultures for assessing toxicologic effects.
    • To demonstrate methods for analyzing synaptic development and function in vitro.
    • To investigate the impact of acute and chronic exposures on neuronal systems.

    Main Methods:

    • Utilizing neuronal cell cultures for toxicological evaluations.
    • Employing morphological and electrophysiological assays for semiquantitative synapse analysis.

    Related Experiment Videos

  • Applying horseradish peroxidase (HPP) for intracellular marker protein injection to correlate structure and function.
  • Measuring neurochemical indices, including transmitter-related enzyme levels.

    • Main Results:

    • Neuronal cell cultures enable detailed morphophysiologic correlations.
    • Synaptic function can be assayed using morphological and electrophysiological techniques.
    • Neurochemical development indices are readily determinable.
    • Examples illustrate acute (opiate) and chronic (inhibitory amino acid) effects on synaptic function.

    Conclusions:

    • Neuronal cell cultures are valuable tools for neurotoxicology research.
    • The model system allows for comprehensive assessment of toxicant impacts on neural development and function.
    • Understanding vulnerability of developing neurons is critical for predicting neurotoxic outcomes.