Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chemical test for mammalian feces: collaborative study

J J Thrasher, J S Gecan

    Journal - Association of Official Analytical Chemists
    |January 1, 1981
    PubMed
    Summary

    A new chemical test accurately detects mammalian feces in food using alkaline phosphatase activity. This method achieved 95% accuracy in identifying fecal samples, enhancing food safety protocols.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Microanalytical Quality of Tomato Products: Juice, Paste, Puree, Sauce and Soup.

    Journal of food protection·2019
    Same author

    Regulatory action criteria for filth and other extraneous materials v. strategy for evaluating hazardous and nonhazardous filth.

    Regulatory toxicology and pharmacology : RTP·2001
    Same author

    Improved reagent for detection of allantoin in thin-layer chromatographic method for urine stains on foods and packaging.

    Journal - Association of Official Analytical Chemists·1988
    Same author

    Improved spray reagent for thin layer chromatographic method for detecting uric acid: collaborative study.

    Journal - Association of Official Analytical Chemists·1986
    Same author

    Extraction and thin layer chromatographic confirmation of urine residues: new plate development.

    Journal - Association of Official Analytical Chemists·1980
    Same author

    Mineral oil extraction of light filth from rubbed sage: collaborative study.

    Journal - Association of Official Analytical Chemists·1979

    Area of Science:

    • Food safety analysis
    • Analytical chemistry methods
    • Enzyme-based detection systems

    Background:

    • Ensuring food safety requires reliable methods to detect contaminants.
    • Mammalian feces can introduce pathogens into food products.
    • Existing detection methods may lack specificity or efficiency.

    Purpose of the Study:

    • To develop and validate a chemical method for identifying mammalian feces in food.
    • To assess the efficacy of alkaline phosphatase as a marker for mammalian fecal contamination.
    • To provide a standardized, accurate test for food analysis.

    Main Methods:

    • A colorimetric assay was developed based on alkaline phosphatase activity.
    • The method involves enzymatic hydrolysis of phenolphthalein diphosphate.
    • Color development in a basic medium quantifies the enzyme's presence.

    Main Results:

    • The chemical method demonstrated high specificity for mammalian alkaline phosphatase.
    • A collaborative study involving 22 animal feces samples achieved 95% correct identification.
    • The assay provides a clear, colorimetric indication of fecal presence.

    Conclusions:

    • The developed chemical method offers a reliable and accurate means for detecting mammalian feces in food.
    • The method's adoption as official first action signifies its validated performance.
    • This assay contributes to improved food safety and quality control measures.

    Related Experiment Videos