Related Experiment Videos
Cycloheximide resistance of Physarum polycephalum
Journal of Bacteriology
|August 1, 1980
Summary
Cycloheximide
Area of Science:
- Cell Biology
- Biochemistry
- Molecular Biology
Background:
- Cycloheximide inhibits protein synthesis in wild-type Physarum polycephalum.
- This inhibition affects DNA synthesis and nucleoside metabolism.
Purpose of the Study:
- To investigate the role of protein synthesis in cycloheximide's effects.
- To characterize cycloheximide resistance in Physarum polycephalum.
Main Methods:
- Isolation and characterization of a cycloheximide-resistant (Cycr) mutant strain.
- Construction of Cycr plasmodial strains and heterokaryons.
- In vitro ribosome resistance assays and biochemical parameter analysis.
Main Results:
- Cycr plasmodia exhibited resistance to cycloheximide's biochemical effects.
- Heterozygous and heterokaryotic strains showed dose-dependent responses.
- Ribosomes from Cycr strains were resistant to cycloheximide in vitro.
Conclusions:
- Cycloheximide's impact on DNA synthesis and nucleoside metabolism is secondary to its protein synthesis inhibition.
- Protein synthesis is a primary target of cycloheximide in Physarum polycephalum.