Characterization of collagens synthesized by cultured bovine corneal endothelial cells

Collagen is the major matrix protein produced by corneal endothelial cell cultures and represented 1% of total protein synthesis. The different types of collagen were determined by a combination of DEAE- and CM-cellulose column chromatography using known internal markers. Type III collagen was the major component both deposited in the extracellular matrix and secreted into the media. The basement membrane collagens, types IV and V, were also found in each compartment, although the latter was associated preferentially with the cell matrix. The ratios of types I:III:IV + V collagens synthesized by corneal endothelial cells were 3:16:1.