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Related Experiment Videos

X-linked processes in serially passaged "aging" human diploid cells

M A Paz, M Torrelio, P M Gallop

    Journal of Gerontology
    |March 1, 1981
    PubMed
    Summary
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    Cellular aging in human diploid fibroblasts involves a declining ratio of hypoxanthine-guanine phosphoribosyltransferase (HGPRT) to adenine phosphoribosyltransferase (APRT) enzyme activities. This ratio can serve as a biomarker for biological age in cultured cells.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Genetics

    Background:

    • Human diploid fibroblasts exhibit reduced proliferation and phenotypic changes with serial passaging in vitro.
    • The purine salvage pathway enzymes, hypoxanthine-guanine phosphoribosyltransferase (HGPRT) and adenine phosphoribosyltransferase (APRT), are crucial for cellular metabolism.
    • Changes in enzyme activity ratios can occur during cellular aging.

    Purpose of the Study:

    • To investigate the changes in the ratio of HGPRT to APRT enzyme activities during serial culture of human diploid fibroblasts.
    • To explore the potential of this enzyme activity ratio as a marker for cellular senescence and biological age.
    • To understand the implications of X-linked gene expression variations in cellular aging.

    Main Methods:

    • Serial in vitro culture of human diploid fibroblasts.

    Related Experiment Videos

  • Assay of hypoxanthine-guanine phosphoribosyltransferase (HGPRT) and adenine phosphoribosyltransferase (APRT) enzyme activities.
  • Calculation of the HGPRT/APRT enzyme activity ratio at different passage levels.

    • Main Results:

    • A progressive decline in the HGPRT/APRT enzyme activity ratio was observed with increasing population doublings.
    • The decline in HGPRT activity was more pronounced than APRT activity.
    • The HGPRT/APRT ratio decreased linearly with population doubling, suggesting a correlation with cellular age.

    Conclusions:

    • The HGPRT/APRT enzyme activity ratio serves as a reliable indicator of biological age in serially passaged human diploid fibroblast cultures.
    • The observed changes may be influenced by X-chromosome inactivation in female cells.
    • Studying these variations can enhance understanding of cellular senescence mechanisms and age-related disorders.