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Related Experiment Videos

The third component of complement inhibits human lymphocyte blastogenesis

B W Needleman, J M Weiler, T L Feldbush

    Journal of Immunology (Baltimore, Md. : 1950)
    |April 1, 1981
    PubMed
    Summary

    Human complement component 3 (C3) and its fragments do not stimulate, but inhibit, lymphocyte activation. Cleavage products of C3 regulate immune responses by suppressing lymphocyte proliferation.

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    Expression of C1 esterase inhibitor by the baculovirus expression vector system: preparation, purification, and characterization.

    Protein expression and purification·2001

    Area of Science:

    • Immunology
    • Complement System Biology

    Background:

    • The complement system, particularly complement component 3 (C3), plays a crucial role in immune regulation.
    • Understanding C3's function in lymphocyte activation is vital for comprehending immune responses.

    Purpose of the Study:

    • To investigate the regulatory effects of purified human C3 and its fragments on human peripheral blood lymphocyte activation.
    • To determine if C3 or its breakdown products modulate lymphocyte proliferation in a serum-free environment.

    Main Methods:

    • Utilized a serum-free tissue culture system to assess purified human C3's impact on lymphocyte activation.
    • Investigated dose-dependent effects of C3 and its fragments on mitogen- and antigen-induced lymphocyte proliferation.
    • Evaluated C3's inhibitory effects at different time points and in macrophage-depleted cultures.

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    Main Results:

    • Purified C3 and its trypsin-digested fragments were found to be non-mitogenic.
    • C3 demonstrated dose-dependent inhibition of both mitogen- and antigen-induced lymphocyte proliferation.
    • Inhibitory effects were observed even when C3 was added late in the culture period, suggesting an impact on late activation events.
    • The inhibitory capacity was linked to a small molecular weight fragment of C3, similar to C3a.

    Conclusions:

    • Cleavage products of C3 possess significant inhibitory activity on lymphocyte activation.
    • These C3 fragments may act as negative regulators in the immune response by suppressing lymphocyte proliferation.
    • The findings suggest a complex role for C3 beyond its classical complement functions.