High-performance liquid chromatographic determination of hydrocortisone cypionate: method development and...

A reversed-phase high-performance liquid chromatographic method for hydrocortisone cypionate bulk drug and oral solution was developed that avoids the use of a heated column as described in USP XX. A study of the effect of the organic modifier concentration on the capacity factor suggests that mixed partition and adsorption phenomena are responsible for the retention of several steroids when acetonitrile is used in the mobile phase. Evidence is presented that hydrogen bonding of the solute molecule with silane hydroxyl groups may be responsible for the adsorption.