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Bound antibody in multiple sclerosis brains
Autopsy tissues of white and grey matter from brains of MS patients and controls were homogenized in PBS and centrifuged at 100,000 x g for 1 h. The residues were thoroughly washed with PBS and further extracted at acidic and alkaline pH. The eluates contained a low but significant amount of IgG, indicating that bound IgG was present in MS brains. A greater yield of IgG was obtained with alkaline pH buffer than with acidic pH buffer. In contrast, sections from 2 of 3 neurologic controls and 3 of 4 non-neurologic control failed to show detectable level of bound IgG extractable at acidic pH. These results might suggest the presence of antigen-antibody complexes in MS brains. Isoelectric focusing analysis demonstrated oligoclonal IgG bands in low pH eluates from MS brains and these bands were partly similar to those seen in neutral (unbound) pH brain extracts.
Autopsy tissues of white and grey matter from brains of MS patients and controls were homogenized in PBS and centrifuged at 100,000 x g for 1 h. The residues were thoroughly washed with PBS and further extracted at acidic and alkaline pH. The eluates contained a low but significant amount of IgG, indicating that bound IgG was present in MS brains. A greater yield of IgG was obtained with alkaline pH buffer than with acidic pH buffer. In contrast, sections from 2 of 3 neurologic controls and 3 of 4 non-neurologic control failed to show detectable level of bound IgG extractable at acidic pH. These results might suggest the presence of antigen-antibody complexes in MS brains. Isoelectric focusing analysis demonstrated oligoclonal IgG bands in low pH eluates from MS brains and these bands were partly similar to those seen in neutral (unbound) pH brain extracts.