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Mouse red cell receptors on human hematopoietic cell lines
Journal of Clinical & Laboratory Immunology
|March 1, 1980
Summary
Spontaneous rosetting with mouse red blood cells (MRBC) identifies a B-lymphocyte subset. Most lymphoblastoid cell lines (LCL) exhibit MRBC rosetting, unlike most tumor cell lines, indicating LCL possess MRBC receptors.
Area of Science:
- Immunology
- Hematology
- Cell Biology
Background:
- Spontaneous rosetting with mouse red blood cells (MRBC) is a potential marker for B-lymphocyte subpopulations.
- Investigating hematopoietic cell lines' capacity for MRBC rosetting can elucidate B-cell characteristics.
Purpose of the Study:
- To compare the MRBC rosetting capacity of various hematopoietic cell lines, including malignant and non-malignant types.
- To determine if MRBC rosetting is a characteristic of specific B-lymphocyte subsets.
Main Methods:
- Assessed rosetting formation between MRBC and diverse hematopoietic cell lines.
- Included malignant cell lines (myeloma, leukemia) and non-malignant Epstein-Barr virus (EBV)-carrying lymphoblastoid cell lines (LCL).
Main Results:
- Exceptional malignant cell lines (1 myeloma, 2 leukemia) showed MRBC rosetting.
- The majority of LCL demonstrated significant MRBC binding.
- MRBC rosetting in LCL was not exclusively dependent on the presence of surface immunoglobulin M (S-IgM).
Conclusions:
- Established lymphoblastoid cell lines (LCL) possess MRBC receptors, indicating a specific B-lymphocyte subset capable of MRBC rosetting.
- Most hematopoietic tumor cell lines generally lack MRBC receptors, even those expressing other B-lymphocyte markers.