[Microbiological assay method of cefadroxil in biological specimens (author's transl)]

A microbiological method for quantitative determination of cefadroxil in biological specimens is described. This method is essentially a cylinder-plate or a paper-disc method using Micrococcus luteus ATCC 9341 as the test organism grown in the tryptosoya broth added with 1.5% agar. Cefadroxil standard calibration curves are prepared in pooled human serum, moni-trol I or consera for the determination of serum level of human, and 0.1 M phosphate buffer (pH 6.0) to determine urine level. Cefadroxil in human serum and urine specimens can be measured by cylinder-plate method as low as 0.16 approximately 0.31 micrograms/ml and 0.08 approximately 0.16 micrograms/ml, respectively. Further, any active metabolites of cefadroxil were not detected on human and rat urine specimens by bioautography.