Related Experiment Video

Updated: Aug 8, 2026

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study
11:10

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

Published on: June 29, 2016

A monosialoganglioside is a monoclonal antibody-defined antigen of colon carcinoma

J L Magnani, M Brockhaus, D F Smith

    Science (New York, N.Y.)
    |April 3, 1981
    PubMed

    Insights

    Researchers identified a specific monosialoganglioside as the antigen for a monoclonal antibody targeting human colon carcinoma cells. This discovery aids in understanding colon cancer biomarkers and developing targeted therapies.

    Area of Science:

    • Biochemistry
    • Immunology
    • Oncology

    Background:

    • Monoclonal antibodies are crucial tools in cancer research and therapy.
    • Identifying specific antigens on cancer cells can lead to targeted treatment strategies.
    • Human carcinoma of the colon presents a significant area for biomarker discovery.

    Purpose of the Study:

    • To identify the specific antigen recognized by a monoclonal antibody directed against human colon carcinoma cells.
    • To characterize the molecular nature of the target antigen for potential therapeutic applications.

    Main Methods:

    • Direct binding assays were performed using the monoclonal antibody on thin-layer chromatograms.
    • Total lipid extracts from colon carcinoma tissues were analyzed.
    • Autoradiography with iodine-125-labeled F(ab')2 fragments was used to detect antibody binding.

    Main Results:

    • The monoclonal antibody specifically bound to a monosialoganglioside.
    • This monosialoganglioside was identified as the antigen present on human colon carcinoma cells.
    • The binding was confirmed through direct antibody interaction with the lipid extracts on chromatograms.

    Conclusions:

    • The antigen for the colon carcinoma-specific monoclonal antibody is a monosialoganglioside.
    • This finding provides a specific molecular target for further research in colon cancer diagnostics and therapeutics.

    More Related Videos

    Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody07:36

    Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody

    Published on: May 16, 2020

    Multiplex Cyclic Fluorescent Immunohistochemistry04:21

    Multiplex Cyclic Fluorescent Immunohistochemistry

    Published on: January 26, 2024

    Related Concept Videos

    Metastasis02:30

    Metastasis

    Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
    Epithelial-to-Mesenchymal Transition
    The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
    Cancer Stem Cells and Tumor Maintenance02:40

    Cancer Stem Cells and Tumor Maintenance

    Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
    Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
    Targeted Cancer Therapies02:57

    Targeted Cancer Therapies

    The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
    There are several types of targeted therapies against specific...
    Combination Therapies and Personalized Medicine02:50

    Combination Therapies and Personalized Medicine

    Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
    The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
    Cancer-Critical Genes II: Tumor Suppressor Genes01:05

    Cancer-Critical Genes II: Tumor Suppressor Genes

    Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
    When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
    Such genes that act...
    Tumor Immunotherapy01:27

    Tumor Immunotherapy

    Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.