Cell transformation of BHK 21 C 13 cells by various chemicals with and without S-9 mix

Transformation of BHK 21 C 13 cells was evaluated by the ability of transformed cells to reproduce in semi solid agar (anchorage independent growth). The in vitro test system was carried out according to Styles with modifications. All compounds were tested with and without metabolic activation (S-9 mix). The results demonstrate the importance of a metabolizing system in this cell transformation assay.