[Method for estimating data of potentiometric titration of membrane fragments by computers]

The flow chart of the computer programme is described for estimation of the quantity and pK values of separate ionogenic sites of the membrane based on the data of potentiometric titration with application of graphic method for calculation of parametres of titration differential curves. Its application makes it possible to reduce the time for calculating the potentiometric curves, to chose minima in the differential curve more objectively for characterization the pK values and to calculate areas of its different plots, which characterize binding capacity for ionogenic groups. The programme was written in the Analytic language for the MIR-2 computer.