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Reovirus-specific messenger ribonucleoprotein particles from HeLa cells
Zeitschrift Fur Naturforschung. Section C, Biosciences
|November 1, 1980
Summary
Reovirus infection at 43°C releases viral messenger RNA (mRNA) from polysomes, forming cytoplasmic messenger ribonucleoprotein particles (mRNPs). These mRNPs contain unique proteins and distinct structures, offering insights into viral RNA regulation.
Area of Science:
- Molecular Biology
- Virology
- Cell Biology
Background:
- Reovirus infection impacts host cell machinery.
- Viral RNA processing and localization are crucial for replication.
Purpose of the Study:
- To characterize the messenger ribonucleoprotein particles (mRNPs) formed by reovirus infection.
- To investigate the composition and structure of reovirus-induced mRNPs.
Main Methods:
- Incubation of reovirus-infected Hela cells at 43°C.
- Isolation and characterization of cytoplasmic messenger ribonucleoprotein particles (mRNPs) via sedimentation and buoyant density centrifugation.
- Protein analysis using molecular mass determination.
- Electron microscopy for structural examination.
Main Results:
- Viral-specific messenger RNA (mRNA) is released from polysomes and accumulates as cytoplasmic mRNPs.
- These reovirus mRNPs exhibit a sedimentation rate of approximately 50S and a buoyant density of 1.42 g/cm³.
- Reovirus mRNPs contain all three size classes of viral mRNA, host cell proteins, and two additional proteins (70 kDa and 110 kDa).
- Electron microscopy revealed unique Y-shaped structures within the reovirus mRNP fraction.
Conclusions:
- Reovirus infection induces the formation of distinct mRNPs containing viral RNA and specific proteins.
- These findings shed light on the mechanisms of viral mRNA regulation and cytoplasmic localization during reovirus infection.