Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Creatine transport into red blood cells

I Syllm-Rapoport, A Daniel, S Rapoport

    Acta Biologica Et Medica Germanica
    |January 1, 1980
    PubMed
    Summary

    Human red blood cells utilize two creatine transport systems: an active, high-affinity process and a low-affinity exchange diffusion. Cellular creatine levels remained stable, with a daily exchange of 20%.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    An algorithm for heterogeneous wireless network connections for user preferences and services.

    Scientific reports·2025
    Same author

    White shark optimizer with optimal deep learning based effective unmanned aerial vehicles communication and scene classification.

    Scientific reports·2023
    Same author

    Improved graph neural network-based green anaconda optimization for segmenting and classifying the lung cancer.

    Mathematical biosciences and engineering : MBE·2023
    Same author

    Effect of Chronic Kidney Disease on Taste Function: A Case Control Study among Nigerian.

    Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India·2023
    Same author

    Paediatric paranasal sinus fibrous dysplasia.

    Tropical doctor·2022
    Same author

    Effect of Chronic Kidney Disease on Olfactory Function: A Case-Control Study.

    Ear, nose, & throat journal·2021

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Physiology

    Background:

    • Creatine is vital for cellular energy metabolism.
    • Understanding creatine transport into red blood cells is crucial for its cellular uptake and energy supply.

    Purpose of the Study:

    • To investigate the mechanisms and kinetics of creatine permeation into human red blood cells.
    • To identify different transport systems involved in creatine uptake.

    Main Methods:

    • Utilized 1-[14C]-creatine to trace creatine movement.
    • Analyzed kinetic data (Vmax and Km) at varying plasma creatine concentrations.
    • Assessed the effect of pH on creatine transport.

    Main Results:

    • Identified two distinct creatine transport systems with different affinities and capacities.
    • The high-affinity transport (Km = 0.087 +/- 0.032 mM) is suggested to be active transport.
    • Low-affinity transport was characterized as exchange diffusion, with minimal pH influence (pH 6.9-7.9).

    Conclusions:

    • Human red blood cells possess dual mechanisms for creatine uptake.
    • Active transport and exchange diffusion contribute to creatine homeostasis within red blood cells.
    • Cellular creatine exhibits a daily exchange rate of 20% with a half-life of approximately 2.5 days.

    Related Experiment Videos