Albumin extravasation in bicuculline-induced blood-brain barrier dysfunction. A comparison between endogeneous and 125I-labeled exogeneous serum albumin

The extravasation of endogeneous rat albumin and exogeneous 125I-labeled human serum albumin was compared in rats subjected to bicuculline-induced blood-brain barrier dysfunction. The correlation between rocket immunoelectrophoretic assays of endogeneous rat albumin and 125I-labeled human serum albumin, assayed by gamma scintillation counting, was good respective of whether 125I-labeled albumin was studies in whole brain tissue or in brain homogenates. The ratio of brain to serum albumin was similar with the two assay methods.