Thymidine incorporation by human corneal endothelium during organ culture

Nine corneas from 5 human adult donors were obtained 11 to 31 h post mortem. In 4 corneas the endothelium was wounded by freezing and in 1 cornea by mechanical means. Care was taken to minimize endothelial damage in the remaining 4 corneas. The corneas were incubated at 31 degrees C for 6 days in a tissue culture medium containing 3H thymidine. Autoradiographs were made of the flat preparations of the endothelium. All corneas contained radioactive endothelial cell nuclei, with the highest concentration of labelled nuclei being in the wound areas. The greatest number of labelled cells was found in the cornea from the youngest donor, 19 years of age, but thymidine uptake also occurred in the oldest cornea, 89-years-old, which additionally had signs of endothelial dystrophy.