[Use of the aliphatic amine reaction with 2,5-dinitrophenol in antibiotic analysis]

Possible use of dinitrophenol reaction with amines in quantitative determination of amines and amino sugars in various antibiotics was investigated. It was found that the reaction with 2,5-dinitrophenol (2,5-DNP) in dimethylsulfoxide (DMSO) providing the highest batochromic shift in the electron spectra was most suitable. Selectivity of the 2,5-DNP reaction with respect to mycosamine contained in polyenic antibiotics was shown. With the method of PMR it was demonstrated that DMSO formed a complex with 2,5-DMP. The electron spectral of the complexes of glucosamine, N-methylgycosamine and levorin with 2,5-DNP were used for calculation of the complex stability constants, which were practically the same. This provided the use of glucosamine as a standard for plotting the calibration curves. A quantitative method for determination of amines in polyenic antibiotics based on the above reaction was developed.